High Performance Liquid Chromatography (HPLC)
HPLC is a very powerful analytical tool which selectively measures chemical constituents, whether active or marker compounds such as Flavonoids, Polyphenols, and Alkaloids. A fingerprint or botanical profile is generated to insure that all constituents are present in the proper ratio.
Thin Layer Chromatography (TLC)
This is a separation technique used to verify the presence of actives and constituents in herbs and botanicals. The botanical in question is separated into its unique chemical spectrum-represented in colored bands, known as a chromatogram. Each band in the chromatogram represents an active or constituent. For proper identification, the chromatogram is then compared to that of a standard, as well as to a previously established reference for that particular botanical.
Fourier Transform Infrared Spectroscopy (FTIR)
This is an analytical spectroscopic technique that utilizes infrared radiation to provide functional information about our botanicals. For each botanical, the spectra record displays the absorption characteristics of functional groups, particularly in the fingerprint region, a singular marker area in each compound. For identification purposes, the spectrum of the botanical in question is overlaid with an established IR spectrum for that particular species.